Journal: Journal of cell science

Article Title: Visualizing extravasation dynamics of metastatic tumor cells.

doi: 10.1242/jcs.069443

Figure Lengend Snippet: Fig. 2. Tumor cells arrest in small vessels then actively migrate along the luminal surface of the vascular endothelium. (A)MDAwt cell expressing CFP and migrating within the ISV lumen for the indicated times (supplementary material Movie 1). Right panel shows 3D isosurface rendering of the tumor cell body for each time point (supplementary material Movie 2). (B)MDAtwist cell expressing RFP and migrating in the lumen of an ISV for the indicated times (supplementary material Movie 4). Right panel shows 3D isosurface rendering of the tumor cell body for each time point (supplementary material Movie 5). (C)Multi-color confocal images of MDAwt and MDA1KO cells labeled with CFP or RFP that have arrested in the ISV lumen. Lower panel shows a control 10m fluorescent Sepharose bead (yellow) arrested in the ISV. (D)Quantification of distance from tumor cell to vessel wall for MDAwt, MDA1KO cells, and Sepharose beads as shown in representative Fig. 2C above. Results are means ± s.e.m. Scale bars: 20m.

Article Snippet: Imaris Contour Surface function was used to manually isolate migrating tumor cell body which was then 3D rendered for each time point using Imaris Isosurface function (Fig. 2A,B).

Techniques: Expressing, Labeling, Control

Journal: Development (Cambridge, England)

Article Title: C. elegans synMuv B proteins regulate spatial and temporal chromatin compaction during development

doi: 10.1242/dev.174383

Figure Lengend Snippet: Loss of synMuv B proteins causes a delay in chromatin compaction in the intestinal lineage during development. (A) Schematic representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. EMS, the precursor cell to the E lineage. (B) Representative images of intestinal nuclei with open and closed extra-chromosomal arrays. In merged image, green is LacI::GFP and red is elt-2p::dTomato. (C) Intestinal nuclei were scored as open or closed based on array morphology (Yuzyuk et al., 2009) in worms raised at 20°C and 26°C at the 8E, 16E, comma and L1 stages. Asterisks represent significant difference between the wild-type population at that stage and temperature (P<0.01, two-way ANOVA). Error bars indicate s.e.m. (D) Representative masked images of intestinal cell nuclei with open or closed extra-chromosomal arrays using the isosurface function in Metamorph; green is LacI::GFP and red is elt-2p::dTomato. (E) The array volume to nuclear volume ratio was measured using the isosurface function in Metamorph at 8E, 16E and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (P<0.01, two-way ANOVA).

Article Snippet: Analysis was performed using Metamorph isosurface function to determine the 3D distance between the two centroids as described by Yuzyuk et al. (2009) .

Techniques:

Journal: bioRxiv

Article Title: C. elegans synMuv B proteins regulate spatial and temporal chromatin compaction during development

doi: 10.1101/538801

Figure Lengend Snippet: (A) A cartoon representing the number of intestinal cells and the approximate number of total cells in the stages scored throughout this study. (B) Large extrachromosomal arrays containing numerous lacO sites represents a pseudo chromosome that when bound by LacI::GFP is used to visualize chromatin morphology. Intestinal nuclei were scored as open or closed based on array morphology . L4 hermaphrodites were placed at 20°C or 26°C and mixed stage F1 embryos were collected and percentage of intestinal nuclei with open arrays at the 8E, 16E, comma, and L1 stage at 20°C and 26°C were scored. 2-way ANOVA was used to determine significance: Asterisks represent significant difference between the wild-type population at that stage and temperature (p<0.01). Error bars indicate standard error of the proportion. n>100 cells (C) 3-D volumetric chromatin compaction analysis was performed using the isosurface function in Metamorph. The volume of the nucleus and the array were measured and used to calculate the percentage of the nucleus that the array encompassed. The percentage of the nucleus contained by the array was plotted at 8E, 16E, and comma at 20°C and 26°C. Each dot represents a single intestinal cell and the line represents the median. Asterisk represents a significant difference between 20°C and 26°C (2-way Anova p<0.01).

Article Snippet: Images were captured on Nikon Confocal and analyzed using Metamorph isosurface function to determine the 3-D distance between the two centroids as described in .

Techniques: